PGC1 alpha + beta Rabbit Polyclonal Antibody

• Catalog Number: orb100963
• Category: Antibodies
• Description: PGC1 alpha + beta Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PGC1 alpha + beta (151-250/798aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 88/113 kDa
• Target: PPARGC1A
• UniProt ID: Q9UBK2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: LEM6; Ligand effect modulator 6; PERC; Peroxisome
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-PGC1 alpha+beta antibody (orb100963), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PGC1 alpha + beta) Polyclonal Antibody, Unconjugated (orb100963) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-PGC1 alpha + beta (orb100963) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88/113 kD, Observed band size: 113 kD.

Sample: Lane 1: Molt-4 (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: Hela (Human) Cell Lysate at 30 ug, Lane 5: Jurkat (Human) Cell Lysate at 30 ug, Lane 6: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-PGC1 alpha + beta (orb100963) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90/113 kD, Observed band size: 113 kD.

Sample: Muscle (Pig) Lysate at 40 ug, Kidney (Mouse) Lysate at 40 ug, Primary: Anti-PGC1 alpha + beta at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 88/113 kD, Observed band size: 92 kD.