Pertuzumab

• Catalog Number: orb1290231
• Category: Antibodies
• Description: Pertuzumab biosimilar antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• CAS Number: 380610-27-5
• Isotype: IgG1-kappa
• Antibody Type: Biosimilar Antibody
• Purity: > 95%
• Conjugation: Unconjugated
• Target: ERBB2/EGFR2/CD340
• Source: CHO cells
• Biological Activity: Pertuzumab (anti-HER2) significantly inhibits the HRG-α-stimulated cellular growth of the 11_18 cells. Pertuzumab blocks HRG-α-stimulated phosphorylation of HER3, mitogen-activated protein kinase (MAPK), and Akt. In contrast, pertuzumab fails to block epidermal growth factor (EGF)-stimulated phosphorylation of EGF receptor (EGFR) and MAPK.Immunoprecipitation shows that pertuzumab inhibited HRG-α-stimulated HER2/HER3 heterodimer formation. HRG-α-stimulated HER3 phosphorylation is also observed in the PC-9 cells co-overexpressing EGFR, HER2, and HER3, but the cell growth is neither stimulated by HRG-α nor inhibited by pertuzumab. Pertuzumab binds to ErbB2 near the center of domain II, sterically blocking a binding pocket necessary for receptor dimerization and signaling. Pertuzumab is specific for human ErbB2 and does not bind to rodent ErbB2 (neu) with detectable affinity.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS buffer pH 7.5
• Alternative names: OMNITARG, rhuMAB 2C4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.