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Catalog Number | orb251532 |
---|---|
Category | Antibodies |
Description | Peroxiredoxin 3/PRDX3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Peroxiredoxin 3 recombinant protein (Position: T110-Q256). Human Peroxiredoxin 3 shares 93% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 3. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 27693 MW |
UniProt ID | P30048 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Thioredoxin-dependent peroxide reductase, mitochon Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Peroxiredoxin 3 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-Peroxiredoxin 3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody.Lane 1:Rat Brain Tissue;2:Mouse Brain Tissue;3:Rat Skeletal Muscle Tissue;4:Mouse Skeletal Muscle Tissue;5:U20S Cell;6:HELA Cell;7:SMMC Cell;8:RH35 Cell;9:A549 Cell.
IF analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody. Peroxiredoxin 3 was detected in immunocytochemical section of U20S cells.
IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody. Peroxiredoxin 3 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.
IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody.Peroxiredoxin 3 was detected in immunocytochemical section of HeLa cell.
IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody.Peroxiredoxin 3 was detected in immunocytochemical section of SMMC-7721 cell.
IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody.Peroxiredoxin 3 was detected in immunocytochemical section of MCF-7 cell.
FC, ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Canine, Equine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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