Perforin antibody

• Catalog Number: orb1151740
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Perforin
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PRF1/7077R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 413-552 was used as the immunogen for the PRF1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PRF1 antibody is available for research use only.
• UniProt ID: P14222
• Storage: Aliquot the PRF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PRF1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free PRF1 antibody (PRF1/7077R) as confirmation of integrity and purity.

IHC staining of FFPE human spleen tissue with PRF1 antibody (clone PRF1/7077R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.