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Catalog Number | orb546391 |
---|---|
Category | Antibodies |
Description | PEF1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human PEF1 recombinant protein (Position: R170-L284). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 30 kDa |
UniProt ID | Q9UBV8 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Peflin; PEF protein with a long N-terminal hydroph Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of PEF1 using anti-PEF1 antibody.Lane 1:human HEK293 cell;2:human placenta tissue;3:human PC-3 cell;4:human U-87MG cell;5:human U-937 cell;6:human HepG2 cell;7;human A431 cell;8;human U2OS cell.
WB analysis of PEF1 using anti-PEF1 antibody.Lane 1:rat lung tissues lysates;2:rat testicular tissue;3:mouse lung tissue;4:mouse testicular tissue.
IHC analysis of PEF1 using anti-PEF1 antibody.PEF1 was detected in paraffin-embedded section of mouse testis tissues.
IHC analysis of PEF1 using anti-PEF1 antibody.PEF1 was detected in paraffin-embedded section of rat testis tissues.
IHC analysis of PEF1 using anti-PEF1 antibody.PEF1 was detected in paraffin-embedded section of human colon cancer tissues.
IHC analysis of PEF1 using anti-PEF1 antibody.PEF1 was detected in paraffin-embedded section of human lung cancer tissues.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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