PDX1 antibody

• Catalog Number: orb1151582
• Category: Antibodies
• Description: Mouse monoclonal antibody to PDX1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-PDX1-2C11
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2c
• Immunogen: Recombinant full-length human PDX1 protein was used as the immunogen for the Pancreas/duodenum homeobox protein 1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Pancreas/duodenum homeobox protein 1 antibody is available for research use only.
• UniProt ID: P52945
• Storage: Aliquot the Pancreas/duodenum homeobox protein 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Pancreas/duodenum homeobox protein 1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using Pancreas/duodenum homeobox protein 1 antibody (green, clone PCRP-PDX1-2C11) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with Pancreas/duodenum homeobox protein 1 antibody (blue, clone PCRP-PDX1-2C11), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free Pancreas/duodenum homeobox protein 1 antibody (clone PCRP-PDX1-2C11) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Pancreas/duodenum homeobox protein 1 antibody (clone PCRP-PDX1-2C11). These results demonstrate the foremost specificity of the PCRP-PDX1-2C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.