PDPK1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1152021
• Category: Antibodies
• Description: PDPK1 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: P1B6
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PDPK1
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100, IHC-F=1:50-100, ICC/IF=1:50, IF=1:50-100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: PDPK1
• UniProt ID: O15530
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 3 phosphoinositide Dependent Protein Kinase 1; hPD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cell line: NIH3T3, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152021.

Tissue:Human liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1152021.

Tissue:Human liver cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1152021.