PDPK1 Antibody

• Catalog Number: orb229750
• Category: Antibodies
• Description: Mouse monoclonal antibody to PDPK1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3H3D9
• Tested applications: FC, ICC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human PDPK1 (AA: 457-556 expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 63.2kDa
• Target: PDPK1
• Entrez: 5170
• UniProt ID: O15530
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: PDK1; PDPK2; PDPK2P; PRO0461
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cells using PDPK1 antibody

Western blot analysis of human recombinant protein using PDPK1 antibody

Western blot analysis of MCF-7 (Lane1), Hela (Lane2), and U937 (Lane3) cell lysate using PDPK1 antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of Hela cells using PDPK1 antibody

Western blot analysis of HEK293 using PDPK1 antibody