PDPK1 Antibody

• Catalog Number: orb229749
• Category: Antibodies
• Description: Mouse monoclonal antibody to PDPK1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7E4G11
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/50 - 1/200; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 63.2kDa
• Target: PDPK1
• Entrez: 5170
• UniProt ID: O15530
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: PDK1; PDPK2; PDPK2P; PRO0461
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MCF-7 (Lane1), Hela (Lane2), K562 (Lane3), U937 (Lane4), A549 (Lane5), NIH/3T3 (Lane6), Jurkat (Lane7), PC-12 (Lane8), and Cos7 (Lane9) cell lysate using PDPK1 antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of A549 cells using PDPK1 antibody

Immunofluorescence analysis of A549 cells using PDPK1 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded bladder cancer tissues using PDPK1 antibody

Western blot analysis of human recombinant protein using PDPK1 antibody

Western blot analysis of HEK293 using PDPK1 antibody