PDL2 Detection Set (Risk Free)

• Catalog Number: orb1227444
• Category: Assays and Kits
• Description: PDL2 Detection Set (Risk Free)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Mouse monoclonal PD-L2 antibodies were raised against the extracellular domain of human PD-L2.
• Concentration: Antibody 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PBS containing 0.02% sodium azide.
• Note: For research use only
• Expiration Date: Please enquire.

Western blot analysis of PD-L2 in overexpressing 293 cells using orb1239853, orb1239856, orb1239859, orb1238786, and orb1239860 antibody at 0.5 and 1 μg/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L2.

Immunocytochemistry of PD-L2 in overexpressing 293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 5 μg/ml.

Immunofluorescence of PD-L2 in overexpressing 293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 20 μg/ml.

Immunohistochemistry of PD-L2 in human colon carcinoma tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 2 μg/ml.

Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.

Titration curve analysis of PD-L2 mAbs to detect recombinant PD-L2 in ELISA with orb1239853, orb1239856, orb1239859, orb1238786, and orb1239860 abs at decreasing concentrations.

Immunofluorescence of PD-L2 in human tonsil tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 20 μg/ml.

Immunohistochemistry of PD-L2 in human tonsil tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 2 μg/ml.