PDL2 Antibody [4E10]

• Catalog Number: orb1239853
• Category: Antibodies
• Description: PDL2 Antibody [4E10]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: [4E10]
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: PD-L2 antibody was raised against the extracellular domain of human PD-L2.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 30 kDa Observed: 38 kDa
• Target: PDCD1LG2
• UniProt ID: P9BQ51
• NCBI: NP_079515
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PD
• Note: For research use only
• Application notes: PD-L2 antibody can be used for detection of PD-L2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml

Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.

Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL. Green: PDL2 Antibody [4E10] (orb1239853) Blue: DAPI staining

Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL. Green: PDL2 Antibody [4E10] (orb1239853) Blue: DAPI staining

Immunofluorescence of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 20 μg/mL. Green: PDL2 Antibody [4E10] (orb1239853) Blue: DAPI staining

Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.

Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.

Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.