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Catalog Number | orb1227731 |
---|---|
Category | Proteins |
Description | PDGF-B Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His Tag & Avi Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
MW | 15 kDa |
Target | PDGF-B |
UniProt ID | P01127 |
Protein Sequence | Ser 82 - Thr 190 |
Source | E. coli |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human PDGF-BB, His Tag at 5 ug/mL (100 ul/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.02-1.2 ug/mL. |
NCBI | AAH29822 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.085% TFA in 30% ACN |
Alternative names | PDGFBB,PDGFB,FLJ12858,PDGF2,SIS,SSV,c-sis Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for E, WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Human PDGF-BB Protein, His Tag, Avi Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Greater than 85% as determined by SDS-PAGE. | |
28.3 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
14.3 kDa | |
E.coli |
Greater than 98% as determined by SDS-PAGE. | |
12.42 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
13.4 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |