PDE4B Antibody

• Catalog Number: orb1247106
• Category: Antibodies
• Description: PDE4B Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-DIDIATEDKSPVDT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 85kDa band observed in Mouse Brain lysates (calculated MW of 83.3kDa according to Human NP_002591.2 and 82.1kDa according to Mouse NP_062814.2). In transfected HEK293 transiently expressing Human PDE4B a band of approx. 85kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.0 ug/ml.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PDE4B
• UniProt ID: Q07343
• NCBI: NP_001032416.1, NP_001032417.1, NP_002591.2, NP_001032418.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PDE4B, phosphodiesterase 4B, cAMP-specific (phosph
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 85kDa band observed in Mouse Brain lysates (calculated MW of 83.3kDa according to Human NP_002591.2 and 82.1kDa according to Mouse NP_062814.2). In transfected HEK293 transiently expressing Human PDE4B a band of approx. 85kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.0 ug/ml.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247106 (0.5 ug/ml) staining of Mouse Brain Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Human PDE4B and probed with orb1247106 (mock transfection in first lane).

orb1247106 (5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.