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Catalog Number | orb344433 |
---|---|
Category | Antibodies |
Description | Pdcd4 (phospho-S457) antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 9G6 |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG1 |
Immunogen | This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q53EL6 |
NCBI | 21735596 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-Pdcd4 pS457 Antibody, Death up-regulate Read more... |
Note | For research use only |
Application notes | Pdcd4 phospho S457 Antibody is tested by ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. |
Expiration Date | 12 months from date of receipt. |
Biorbyt Antibody orb344433 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Western blot using Biorbyt Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1° Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4°C in 1% BSA-TBS-T) followed by 2° Ab Peroxidase Conjugated Rabbit anti-Ms CUST10M at 1:40000 in orb348637 30 min RT. Bands at ~62 kD and ~32 kD were detected.
Western blot using Biorbyt's Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells (p/n orb348723) after 5 min treatment with 30 ng/mL PDGF [lane 2]. No reactivity is seen for (non-phosphorylated) in unstimulated NIH 3T3 cells (p/n orb348714) [lane 1]. The membrane was probed with the primary antibody at a 1:2000 dilution, overnight at 4°C. For detection HRP conjugated Rb-a-Mouse IgG (p/n orb347544) was used at a 1:20000 dilution in blocking buffer (p/n orb348637) for 1 h at 4°C.
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IP, WB | |
Human, Mouse, Rat, Xenopus | |
Mouse | |
Monoclonal | |
Biotin |
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