PDCD1LG2 Mouse Monoclonal Antibody

• Catalog Number: orb783343
• Category: Antibodies
• Description: PDCD1LG2 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: G8F6
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,Κ
• Immunogen: KLH conjugated synthetic peptide derived from human PDCD1LG2 (31-130/273aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:1000-2000, IHC-P=1:100-400, IHC-F=1:100-400, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: PDCD1LG2
• UniProt ID: Q9BQ51
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PD L2; PD-L2; PD1L2_HUMAN; B7 dendritic cell molec
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of PDL-2 in untransfected (left) or transfected (right) with 293T cell sections. Cell was fixed with formaldehyde, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Sample: Lane 1: Non-transfected 293T cell lysates, Lane 2: Transfected PD-L2-transfected 293T cell lysates, Primary: Anti-PDCD1LG2 (orb783343) at 1/8000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 40-55 kD.