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Catalog Number | orb1937580 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31095 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000-1:2000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 30957 Da |
Target | This PDCD1LG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-75 amino acids from the N-terminal region of human PDCD1LG2. |
UniProt ID | Q9BQ51 |
NCBI | NP_079515.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Programmed cell death 1 ligand 2, PD-1 ligand 2, P Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-PDCD1LG2 Antibody (N-term) at 1:1000-1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Raji whole cell lysate. Lane 4: ZR-75-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
PDCD1LG2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PDCD1LG2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
PDCD1LG2 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35 ug/lane). This demonstrates the PDCD1LG2 antibody detected the PDCD1LG2 protein (arrow).