PDCD1LG2 Antibody (N-term)

• Catalog Number: orb1925355
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2011CT241.19.54
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,κ
• Dilution range: WB: 1:4000, IHC-P: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 30957 Da
• Target: This PDCD1LG2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 40-75 amino acids from the N-terminal region of human PDCD1LG2.
• UniProt ID: Q9BQ51
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Programmed cell death 1 ligand 2, PD-1 ligand 2, P
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PDCD1LG2 Antibody (N-term) at 1:4000 dilution. Lane 1: Non-transfected 293T whole cell lysate. Lane 2: Transfected PD-L2-transfected 293T whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of PDL-2 in untransfected (left) or transfected (right) with 293T cell sections. Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1: 25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.