PDCD1 Antibody / PD-1 / PD1

• Catalog Number: orb1823708
• Category: Antibodies
• Description: PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PDCD1/4231R
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant fragment corresponding to the extracellular domain of human PDCD1 protein was used as the immunogen for the PDCD1 antibody.
• Dilution range: ELISA (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This PDCD1 antibody is available for research use only.
• UniProt ID: Q15116
• Storage: Aliquot the PDCD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node tissue with PD1 (CD279) antibody (clone PDCD1/4231R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with PD1 (CD279) antibody (clone PDCD1/4231R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.