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Catalog Number | orb1239763 |
---|---|
Category | Antibodies |
Description | PDCD1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 7H6 |
Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | PD-1 antibody was raised against the extracellular domain of human PD-1. |
Concentration | 1 mg/mL |
Dilution range | PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 32 kDa Observed: 38 kDa |
Target | PDCD1 |
UniProt ID | Q15116 |
NCBI | NP_005009 |
Storage | PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol. |
Alternative names | PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD Read more... |
Note | For research use only |
Application notes | PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at 1 μg/mL.
Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 μg/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 μg/mL.
Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 μg/mL.
Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.
Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 μg/mL. Red: PD1 Antibody [7H6] (orb1239763) Blue: DAPI staining
Western blot analysis of PD-1 in overexpressing 293 cells using orb1239745, orb1239769, and orb1239763 antibody at 1, 0.5, and 0.25 μg/ml, respectively.
Flow cytometry analysis of PD-1 overexpressing 293 cells using orb1239763 at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.
Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with orb1239745, orb1239769, orb1239763, orb1239738, and orb1239734 abs at decreasing concentrations.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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