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Catalog Number | orb1227446 |
---|---|
Category | Assays and Kits |
Description | PD1 Detection Set (Risk Free) |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Mouse monoclonal PD-1 antibodies were raised against the extracellular domain of human PD-1. |
Concentration | Antibody 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | PBS containing 0.02% sodium azide. |
Note | For research use only |
Expiration Date | Please enquire. |
Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with orb1239745, orb1239769, orb1239763, orb1239738, orb1239734, and orb1239755 antibodies at decreasing concentrations.
Western blot analysis of PD-1 in over expressing HEK293 cells using orb1239745, orb1239769, and orb1239763 antibodies at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml.
Immunocytochemistry of PD-1 in over expressing HEK293 cells using (A) orb1239745, (B) orb1239769, (C) orb1239763, (D) orb1239738, (E) orb1239734, (F) orb1239755, and (G) control mouse IgG antibody at 10 μg/ml.
Immunofluorescence of PD-1 in over expressing HEK293 cells using (A) orb1239745, (B) orb1239769, (C) orb1239763, (D) orb1239738, (E) orb1239734, (F) orb1239755, and (G) control mouse IgG antibody at 20 μg/ml.
Immunofluorescence of PD-1 in human lymph node tissue using (A) orb1239745, (B) orb1239769, (C) orb1239763, (D) orb1239738, (E) orb1239734, (F) orb1239755, and (G) control mouse IgG antibody at 20 μg/ml.
Flow cytometry analysis of PD-1 in over expressing HEK293 cells using (A) orb1239763, (B) orb1239738, (C) orb1239734, (D) orb1239755, and (E) mouse IgG at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: PD-1 over expressing HEK293 cells.
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