PD-L2 Antibody / Programmed death ligand 2 / CD273

• Catalog Number: orb607156
• Category: Antibodies
• Description: Recognizes a protein identified as PD-L2 (same as PDCD1LG2). Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator). The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PDL2/2676
• Tested applications: ELISA, FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant fragment of human PD-L2 protein within amino acids 27-220 was used as the immunogen for the PD-L2 antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PD-L2 antibody is available for research use only.
• UniProt ID: Q9BQ51
• Storage: Store the PD-L2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PD-L2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human Jurkat cells with PD-L2 antibody (green, clone PDL2/2676) and Reddot nuclear stain (red).

Western blot testing of 1) human Raji, 2) human HepG2, 3) human ThP-1, 4) mouse RAW264.7, 5) mouse NIH-3T3 and 6) human U-87 MG cell lysate with PD-L2 antibody (clone PDL2/2676). Expected molecular weight: 31-60 kDa depending on glycosylation level.

Flow cytometry testing of human Jurkat cells with PD-L2 antibody (clone PDL2/2676); Red=isotype control, Blue= PD-L2 antibody.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PD-L2 antibody (clone PDL2/2676). These results demonstrate the foremost specificity of the PDL2/2676 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PD-L2 antibody (clone PDL2/2676) as confirmation of integrity and purity.