PD-L2 Antibody / Programmed death ligand 2 / CD273

• Catalog Number: orb607123
• Category: Antibodies
• Description: PD-L2/PDCD1LG2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CDLA273-1
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant fragment of human PD-L2 protein within amino acids 27-220 was used as the immunogen for the PD-L2 antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PD-L2 antibody is available for research use only.
• UniProt ID: Q9BQ51
• Storage: Store the PD-L2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PD-L2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of 1) human Raji and 2) mouse RAW264.7 cell lysate with PD-L2 antibody (clone CDLA273-1). Expected molecular weight: 31-60 kDa depending on glycosylation level.

Flow cytometry testing of human Jurkat cells with PD-L2 antibody (clone CDLA273-1); Red=isotype control, Blue= PD-L2 antibody.

Immunofluorescent staining of human Jurkat cells with PD-L2 antibody (green, clone CDLA273-1) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.