PD-L1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb704273
• Category: Antibodies
• Description: PD-L1 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 8C14
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PD-L1
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1000, IHC-P=1:20-100, IHC-F=1:20-100, IF=1:20-100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33 kDa
• Target: CD274
• UniProt ID: Q9NZQ7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Programmed cell death 1 ligand 1; CD274; B7 H; B7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (orb704273) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (orb704273) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (orb704273) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Placenta (Mouse) Lysate at 40 ug, Lane 3: Jurkat (Human) Cell Lysate at 30 ug, Lane 4: MDA-MB-231 (Human) Cell Lysate at 30 ug, Lane 5: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-PD-L1 (orb704273) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 45 kD.