PD L1 Monoclonal Antibody (PDL1)

• Catalog Number: orb1787836
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:600, IHC-P: 1:100-1:600, IHC-P-Leica: 1:100-1:600, IHC-P: 1:100-1:600, IHC-P: 1:100-1:600, IHC-P: 1:100-1:600
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 33275 Da
• Target: This PD L1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 256-290 amino acids from the human region of human PD L1.
• UniProt ID: Q9NZQ7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Programmed cell death 1 ligand 1, PD-L1, PDCD1 lig
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of PDL-1 in human tonsil tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Dako test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemical analysis of PDL-1 in human tonsil tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by abgent test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Dako test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Leica test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by abgent test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemical analysis of PDL-1 in MCF-7 cell (left) and NCI-H226 (right) cell sections by abgent test.Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-PDL-1 Antibody at 0.5-1µg/ml dilution. Lane 1: 293T/PDL1 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: NCI-H1975 whole cell lysate. Lane 4: MDA-MB231 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lane 6: NCI-H226 whole cell lysate. Lysates/proteins at 30 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjµgated at 1/5000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.