PD L1 antibody

• Catalog Number: orb1294268
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PD L1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human PD-L1 / CD274
• Dilution range: Western Blot & Immunoprecipitation: Reported in the literature, not verified in house. Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin) 1:100-1:250
• Conjugation: Unconjugated
• Target: PD-L1 / CD274 Intracellular domain (IHC prefer)
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1/CD274 with orb1294268 at 1/150.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1/CD274 with orb1294268 at 1/150.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1/CD274 with orb1294268 at 1/150.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. stained with orb1294268 Anti-PD-L1/CD274 antibody (red) at 1:200 and 4°C.

Customer Feedback: Cell: MDA-MB-231; 1Ab: orb1294268 1-500; 2Ab: anti Rabbit IgG (Light chain) 1-3000.