PD-L1 antibody

• Catalog Number: orb1152461
• Category: Antibodies
• Description: Human monoclonal antibody to PD-L1
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: 332M7
• Tested applications: Blocking, FC
• Reactivity: Human, Monkey, Mouse
• Isotype: IgG1
• Immunogen: The original parental antibody 332M1 was generated by immunizing BALB/c mice with recombinant human PD-L1. Later on the humanized version of 332M1 was generated (332M7) by grafting the CDRs of the original mouse antibody onto human framework regions.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: PD-L1
• UniProt ID: Q9NZQ7
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PD
• Note: For research use only
• Application notes: The binding characterization of this antibody towards human, mouse and cynomolgus monkey PD-L1 was done using flow cytometry. This antibody was shown to block the binding of hPD-1-Fc protein to hPD-L1 expressed on the surface of the HEK-293T cells at a concentration of 10 μg/ml. The mouse version of this antibody was reported to inhibit patient derived melanoma tumor in tumor-bearing mice. There was also found an increase in the gene expression of CD8 and IFN-γ in tumor samples from mice treated with mouse version of this antibody (US20180346571).
• Expiration Date: 12 months from date of receipt.