PD-L1 Antibody / B7-H1 / CD274

• Catalog Number: orb639693
• Category: Antibodies
• Description: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PDL1/2743
• Tested applications: ELISA, FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 39-191 from the human protein was used as the immunogen for this PD-L1 antibody.
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PD-L1 antibody is available for research use only.
• UniProt ID: Q9NZQ7
• Storage: Store the PD-L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of human Jurkat cells with PD-L1 antibody (clone PDL1/2743); Red=isotype control, Blue= cells alone, Orange= PD-L1 antibody.

Immunofluorescent staining of human Jurkat cells with PD-L1 antibody (clone PDL1/2743, green) and Reddot nuclear stain (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PD-L1 antibody (clone PDL1/2743). These results demonstrate the foremost specificity of the PDL1/2743 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/2743) as confirmation of integrity and purity.