PD-L1 Antibody / B7-H1 / CD274

• Catalog Number: orb1822746
• Category: Antibodies
• Description: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancers ability to evade the immune system.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PDL1/8222R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 190-290) from the human protein was used as the immunogen for the PD-L1 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PD-L1 antibody is available for research use only.
• UniProt ID: Q9NZQ7
• Storage: Aliquot the PD-L1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HDLM-2 cell lysate with PD-L1 antibody (clone PDL1/8222R). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

IHC staining of FFPE human tonsil tissue with PD-L1 antibody (clone PDL1/8222R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human placental tissue with PD-L1 antibody (clone PDL1/8222R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/8222R) as confirmation of integrity and purity.