PCSK9 Antibody

• Catalog Number: orb1247133
• Category: Antibodies
• Description: PCSK9 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: CRSRHLAQASQELQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Adipose lysates (calculated MW of 74.3kDa according to NP_777596.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1;14(9):1161-9, PMID: 15772090. A customer found positive results in WB on Human Liver.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PCSK9
• UniProt ID: Q8NBP7
• NCBI: NP_777596.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PCSK9, FH3, NARC1, HCHOLA3, proprotein convertase
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Adipose lysates (calculated MW of 74.3kDa according to NP_777596.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1;14(9):1161-9, PMID: 15772090. A customer found positive results in WB on Human Liver.
• Expiration Date: 12 months from date of receipt.

orb1247133 (4 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. This data is from a previous batch, not on sale.

orb1247133 (0.3 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.