PCK1 antibody

• Catalog Number: orb136050
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PCK1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 490-622 of human PCK1 (NP_002582.3).
• Concentration: 1 mg/ml
• Dilution range: WB: 1:1000-2000, IHC-P: 1:50-200
• Conjugation: Unconjugated
• MW: 69kDa
• UniProt ID: P35558
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Alternative names: anti MGC22652 antibody, anti PEPCK-C antibody, ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HepG2 cells using PCK1 Rabbit pAb (orb136050) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded human kidney cancer using PCK1 Rabbit pAb (orb136050) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded mouse kidney using PCK1 Rabbit pAb (orb136050) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded rat kidney using PCK1 Rabbit pAb (orb136050) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300 µg extracts of Mouse liver cells using 3 µg PCK1 antibody (orb136050). Western blot was performed from the immunoprecipitate using PCK1 antibody (orb136050) at a dilution of 1:1000.

Western blot analysis of lysates from HepG2 cells, using PCK1 Rabbit pAb (orb136050) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western blot analysis of various lysates using PCK1 Rabbit pAb (orb136050) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.