PBK (phospho-Thr9) antibody

• Catalog Number: orb769666
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PBK (phospho-Thr9)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PBK/TOPK around the phosphorylation site of Thr9. AA range:1-50
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 36
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-PBK antibody, anti-TOPK antibody, anti-Lympho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of 293T cells using Phospho-PBK (T9) Polyclonal Antibody diluted at 1:500.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PBK/TOPK (Phospho-Thr9) Antibody.

Western blot analysis of lysates from K562 cells treated with UV 30', using PBK/TOPK (Phospho-Thr9) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).