PAX6 Antibody

• Catalog Number: orb1294411
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PAX6
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region.
• Dilution range: Western Blot 1:500 Immunofluorescence 1:200–1:300 Immunohistochemistry (Paraffin) 1:100
• Conjugation: Unconjugated
• Target: PAX6
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-PAX6 antibody - 1/500 dilution, Lysates at 60 μg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294411 overnight at 4°C.

Anti-PAX6 antibody - 1/500 dilution, Lysates at 60 μg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294411 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294411 anti-PAX6 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling PAX6 with orb1294411 at 1/400.