PAX5 Antibody

• Catalog Number: orb699704
• Category: Antibodies
• Description: The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rPAX5/4228
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A portion of amino acids 235-382 from the human protein was used as the immunogen for the recombinant PAX5 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant PAX5 antibody is available for research use only.
• UniProt ID: Q02548
• Storage: Store the recombinant PAX5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant PAX5 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil with recombinant PAX5 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with recombinant PAX5 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant PAX5 antibody as confirmation of integrity and purity.