PARP1 Antibody (N-term)

• Catalog Number: orb1930063
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14153
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 113084 Da
• Target: This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-214 amino acids from the N-terminal region of human PARP1.
• UniProt ID: P09874
• NCBI: NP_001609.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosy
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

PARP1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PARP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the PARP1 antibody detected the PARP1 protein (arrow).

Western blot analysis of PARP1 (arrow) using rabbit polyclonal PARP1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2).