Parkin (phospho-Ser65) antibody

• Catalog Number: orb312554
• Category: Antibodies
• Description: Parkin (phospho-Ser65) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Monkey, Porcine, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Parkin around the phosphorylation site of Ser65 (QQ(p-S)IV)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: phospho-Parkin (Ser65)
• UniProt ID: O60260
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Parkin (phospho S65); p-Parkin (phospho S65); AR J
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Chakrabarti, Lina et al. Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability MAbs, 14, 2020081 (2022)

PubMed: 35030984

Huang, Yi-Fan et al. The protective effort of GPCR kinase 2-interacting protein-1 in neurons via promoting Beclin1-Parkin induced mitophagy at the early stage of spinal cord ischemia-reperfusion injury FASEB J, 34, 2055-2074 (2020)

PubMed: 31908016

Yu, Shao-Hua et al. Human antigen R regulates hypoxia-induced mitophagy in renal tubular cells through PARKIN/BNIP3L expressions J Cell Mol Med, 25, 2691-2702 (2021)

PubMed: 33496385

Lane, Sydney L. et al. Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice Int J Mol Sci, 22, 13019 (2021)

PubMed: 34884824

Sun, Xin et al. Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy Acta Pharm Sin B, 12, 838-852 (2022)

PubMed: 35256949

Zhang, Qin et al. Regulation of mitophagy through HIF-1?/miR-140-5p/PARKIN axis in acute kidney injury Environ Toxicol, 37, 1759-1767 (2022)

PubMed: 35312153

Rai, Rajani et al. Distinct mechanism of cervical cancer cell death caused by the investigational new drug SHetA2 Front Oncol, 12, 958536 (2022)

PubMed: 36203464

Singh, Anurag et al. Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults Cell Rep Med, 3, 100633 (2022)

PubMed: 35584623

Chao, Tony et al. Burn-Induced Reductions in Mitochondrial Abundance and Efficiency are More Pronounced with Small Volumes of Colloids in Swine Am. J. Physiol., Cell Physiol., (2019)

PubMed: 31532719

Western blot analysis of A673 Cell Lysate using Parkin antibody

Immunohistochemical staining of human brain glioma using Parkin antibody

Western blot analysis of Lane 1: Cerebrum (Mouse) Lysate at 40 ugLane 2: Lung (Mouse) Lysate at 40 ugLane 3: SH-SY5Y (Human) Cell Lysate at 30 ugLane 4: 293T (Human) Cell Lysate at 30 ugLane 5: MCF-7 (Human) Cell Lysate at 30 ugLane 6: Jurkat (Human) Cell

Western blot analysis of Thymus (Mouse) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugLung (Mouse) Lysate at 40 ugSkeletal muscle (Mouse) Lysate at 40 ug using Parkin (phospho-Ser65) antibody