PARK7/DJ1 Rabbit Polyclonal Antibody

• Catalog Number: orb10543
• Category: Antibodies
• Description: PARK7/DJ1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CAP1 (101-189/189aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 20 kDa
• Target: PARK7
• UniProt ID: Q99497
• RRID: AB_10750152
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PARK7; PARK-7; Parkinson disease protein 7; Park7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-PARK7/CAP1 antibody (orb10543), Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, Primary antibody (orb10543, 0.2 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PARK7) Polyclonal Antibody, Unconjugated (orb10543) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PARK7) Polyclonal Antibody, Unconjugated (orb10543) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: Thyroid gland Rat) Lysate at 40 ug, Lane 5: Kidney (Rat) Lysate at 40 ug, Lane 6: Liver (Rat) Lysate at 40 ug, Lane 7: Hela (Human) Cell Lysate at 30 ug, Lane 8: U937 (Human) Cell Lysate at 30 ug, Lane 9: K562 (Human) Cell Lysate at 30 ug, Lane 10: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-PARK7/DJ1 (orb10543) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

The blue histogram is unstained cells (Hepg2 cells), concentration 1:50, The Wathet Blue histogram is cells stained with secondary antibody alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody, plus secondary antibody. The green histogram is cells stained with Rabbit Anti-PARK7/CAP1 antibody (orb10543) plus secondary antibody.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CAP1/PARK7 Polyclonal Antibody, Unconjugated (orb10543) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CAP1/PARK7 Polyclonal Antibody, Unconjugated (orb10543) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.