PAR4 Rabbit Polyclonal Antibody

• Catalog Number: orb783031
• Category: Antibodies
• Description: PAR4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Proteinase-activated receptor 4 (301-385/385aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: F2RL3
• UniProt ID: Q96RI0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: F2RL3; F2R Like Thrombin/Trypsin Receptor 3; Coagu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Lung tissue lysates, Lane 4: Rat Thyroid gland tissue lysates, Lane 5: Rat Testis tissue lysates, Lane 6: Human K562 cell lysates, Lane 7: Human THP-1 cell lysates, Lane 8: Human Hela cell lysates, Lane 9: Human Huvec cell lysates, Primary: Anti-Proteinase-activated receptor 4/PAR4 (orb783031) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41/38 kD.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PAR4 Polyclonal Antibody, Unconjugated (orb783031) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.