PAPP-A Antibody / Pappalysin-1

• Catalog Number: orb607180
• Category: Antibodies
• Description: Pregnancy Associated Plasma Protein (PAPP-A) is found in maternal blood that increases as pregnancy progresses, although it is not specific to pregnancy. It is principally expressed in the syncytiotrophoblast of the placenta, which forms the main source of circulating maternal PAPP-A. It cleaves insulin-like growth factor binding proteins (IGFBPs), IGFBP-4 and IGFBP-5. IGFBP-4 cleavage is enhanced significantly in the presence of bound IGF, whereas IGFBP-5 cleavage is inhibited slightly by IGF presence. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. PAPPA has also been suggested as a potential biomarker of acute myocardial infarction and Coronary Artery Disease (CAD).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PAPPA/2715
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 351-523 from the human protein was used as the immunogen for the PAPP-A antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PAPP-A antibody is available for research use only.
• UniProt ID: Q13219
• Storage: Store the PAPP-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human placenta with PAPP-A antibody (clone PAPPA/2715). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human placenta with PAPP-A antibody (clone PAPPA/2715). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PAPP-A antibody (clone PAPPA/2715). These results demonstrate the foremost specificity of the PAPPA/2715 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PAPP-A antibody (clone PAPPA/2715) as confirmation of integrity and purity.