Papain antibody (Peroxidase)

• Catalog Number: orb344364
• Category: Antibodies
• Description: Papain antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, IHC: 1:200- 1:1,000, WB: 1:500- 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]. Cross reactivity against Papain from other sources may occur but has not been specifically determined.
• Conjugation: HRP
• UniProt ID: P00784
• NCBI: P00784.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat anti-Papain Antibody HRP Conjugated, Peroxida
• Note: For research use only
• Application notes: Anti-Papain peroxidase antibody has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 ug of Papain [Carica papaya] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-Papain Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: none. Block: orb348637 for 30 min at RT. Predicted/Observed size: 23.4 kDa, > 25 kDa for Papain.