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Papain Antibody Peroxidase Conjugated
Catalog Number: orb344363
| Catalog Number | orb344363 |
|---|---|
| Category | Antibodies |
| Description | Papain antibody (Peroxidase) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, IHC, WB |
| Reactivity | Plant |
| Isotype | IgG |
| Immunogen | Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya. |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:1,000 - 1:5,000, IHC: 1:200- 1:1,000, WB: 1:500- 1:2,500 |
| Form/Appearance | Lyophilized |
| Purity | Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]. Cross reactivity against Papain from other sources may occur but has not been specifically determined. |
| Conjugation | HRP |
| UniProt ID | P00784 |
| NCBI | P00784.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | goat anti-Papain Antibody HRP Conjugated, Peroxida Read more... |
| Note | For research use only |
| Application notes | Anti-Papain peroxidase antibody has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 ug of Papain [Carica papaya] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for applications that are not stated below. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Goat anti-Papain Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: none. Block: orb348637 for 30 min at RT. Predicted/Observed size: 23.4 kDa, >25 kDa for Papain.
