Papain antibody (Biotin)

• Catalog Number: orb344379
• Category: Antibodies
• Description: Papain antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Papain [Carica papaya]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:10,000, IHC: User Optimized, IP: User Optimized, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]. Cross reactivity against Papain from other sources may occur but have not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P00784
• NCBI: P00784.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Papain Antibody biotin Conjugation, biot
• Note: For research use only
• Application notes: Anti-Papain Biotin antibody has been tested by ELISA and western blot. Expect band at 38.9 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-Papain Antibody Biotin Conjugated. Lane 1: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP Streptavidin (p/n orb348767) secondary antibody at 1:40000 for 30 minutes at RT. Block: orb348637 for 30 minutes at RT. Predicted/Observed size: 23 kDa, 23 kDa for Papain.