Pan-HLA Antibody (MHC II)

• Catalog Number: orb640104
• Category: Antibodies
• Description: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rHLA-Pan/3475
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: The non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the Pan-HLA antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Pan-HLA antibody is available for research use only.
• UniProt ID: P01909, P01920, P01903, P04440, P01908
• Storage: Store the recombinant Pan-HLA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) Ramos and 2) spleen tissue lysate with recombinant Pan-HLA antibody (clone rHLA-Pan/3475).

SDS-PAGE analysis of purified, BSA-free recombinant Pan-HLA antibody (clone rHLA-Pan/3475) as confirmation of integrity and purity.

Flow cytometry testing of human Raji cells with recombinant Pan-HLA antibody (clone rHLA-Pan/3475); Red=isotype control, Blue= recombinant Pan-HLA antibody.

IHC testing of FFPE human tonsil tissue with recombinant Pan-HLA antibody (clone rHLA-Pan/3475). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.

Immunofluorescence staining of human Raji cells with recombinant Pan-HLA antibody (green, clone rHLA-Pan/3475) and Reddot nuclear stain (red).