Pan-HLA Antibody (MHC II)

• Catalog Number: orb607160
• Category: Antibodies
• Description: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: HLA-Pan/2967R
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: The non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the Pan-HLA antibody.
• Dilution range: ELISA: 2-5ug/ml (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Pan-HLA antibody is available for research use only.
• UniProt ID: P01909, P01920, P01903, P04440, P01908
• Storage: Store the Pan-HLA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Pan-HLA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of human Raji cells with Pan-HLA antibody (green, clone HLA-Pan/2967R) and Reddot nuclear stain (red).

Immunofluorescence staining of human Ramos cells with Pan-HLA antibody (green, clone HLA-Pan/2967R) and Reddot nuclear stain (red).

IHC testing of FFPE human tonsil tissue with recombinant Pan-HLA antibody (clone HLA-Pan/2967R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to testing.

Flow cytometry testing of human Ramos cells with Pan-HLA antibody (clone HLA-Pan/2967R); Red=isotype control, Blue= Pan-HLA antibody.

Western blot testing of human Ramos lysate with Pan-HLA antibody (clone HLA-Pan/2967R). Expected molecular weight: 33-35 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Pan-HLA antibody (clone HLA-Pan/2967R). These results demonstrate the foremost specificity of the HLA-Pan/2967R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant Pan-HLA antibody (clone HLA-Pan/2967R) as confirmation of integrity and purity.