Pan-Cytokeratin, Pan-CK

• Catalog Number: orb1294309
• Category: Antibodies
• Description: Rabbit polyclonal antibody to pan Cytokeratin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Detects endogenous levels of total keratin 5, 6, 7, 8, 14, 17, 18, 19 and 20. The antibody does not cross-react with other keratins.
• Dilution range: Western Blot 1:2000–1:5000 Immunofluorescence 1:400–1:800 Immunohistochemistry (Paraffin) 1:400–1:800 Flow Cytometry 1:300–1:500
• Conjugation: Unconjugated
• Target: Pan-Cytokeratin, Pan-CK
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C PBS (pH 7.4), 40% Glycerol. 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-pan Cytokeratin antibody - at 1/3000 dilution.

Anti-pan Cytokeratin antibody - at 1/3000 dilution, Lysates at 45 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294309 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294309 anti-pan Cytokeratin0 antibody (red) at 1:300 and 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling pan Cytokeratin with orb1294309 at 1/400.

Product: pan Cytokeratin antibody; Catalog: orb1294309; Cell: MCF-7; pan Cytokeratin (orb1294309): 1-350.

Side by side with VANTANA IVD grade antibody.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294309 anti-pan Cytokeratin antibody (green) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.