Pan Cytokeratin Cocktail antibody

• Catalog Number: orb1151482
• Category: Antibodies
• Description: Mouse monoclonal antibody to Pan Cytokeratin Cocktail
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: Cocktail rPCK/6750
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Crude cytokeratin extract prepared from RT-4 and MCF-7 cells was used as the immunogen for the recombinant Pan-CK antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Pan-CK antibody is available for research use only.
• Storage: Aliquot the recombinant Pan-CK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Pan-CK antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate tissue with recombinant Pan-CK antibody (clone rPCK/6750). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Pan-CK antibody (Cocktail rPCK/6750) as confirmation of integrity and purity.