Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

• Catalog Number: orb640118
• Category: Antibodies
• Description: Anti-cytokeratin clone PCK/3150 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/3150 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/3150 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCK/3150
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A crude cytokeratin extract prepared from RT-4 and MCF-7 cells was used as the immunogen for this Pan Cytokeratin antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Pan Cytokeratin antibody is available for research use only.
• Storage: Store the Pan Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of permeabilized human HeLa cells with Pan Cytokeratin antibody (clone PCK/3150, green) and Reddot nuclear stain (red).

Flow cytometry testing of permeabilized human HeLa cells with Pan Cytokeratin antibody (clone PCK/3150); Red=isotype control, Blue= Pan Cytokeratin antibody.

IHC staining of FFPE human prostate with Pan Cytokeratin antibody (clone PCK/3150). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate with Pan Cytokeratin antibody (clone PCK/3150). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Pan Cytokeratin antibody (clone PCK/3150) as confirmation of integrity and purity.