Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

• Catalog Number: orb1825078
• Category: Antibodies
• Description: MonoPoly antibodies are designed by pooling several monospecific, recombinant monoclonal antibodies against a target. MonoPoly antibodies are a kind of synthetic polyclonal antibodies that can be produced in unlimited quantity with a strict lot-to-lot consistency. These antibodies are highly specific with exquisite sensitivity and a single MonoPoly antibody can be used for a variety of applications.This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 55kDa (CK7); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 46kDa (CK17); 45kDa (CK18), 40kDa (CK19) and 46kDa (CK20). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This antibody is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MonoPoly/4999R
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant fragments and/or synthetic peptides of human Cytokeratin proteins were used as the immunogen for the Pan Cytokeratin antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Pan Cytokeratin antibody is available for research use only.
• UniProt ID: Q7Z794, Q01546
• Storage: Aliquot the Pan Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with Pan Cytokeratin antibody (clone MonoPoly/4999R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human basal cell carcinoma with Pan Cytokeratin antibody (clone MonoPoly/4999R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma tissue with Pan Cytokeratin antibody (clone MonoPoly/4999R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.