Pan-Cytokeratin, 2 Clones (AE1/AE3)

• Catalog Number: orb27182
• Category: Antibodies
• Description: Mouse monoclonal antibody to pan Cytokeratin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AE1/AE3
• Tested applications: IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Human epithelial keratins
• Concentration: 70 µg/ml
• Dilution range: (liquid conc.) 1:20 - 1:50
• Conjugation: Unconjugated
• Hazard Information: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• RRID: AB_10934672
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
• Note: For research use only
• Application notes: Positive Control: Appendix. Pre-Treatment: Paraffin sections may be pre-treated with 1 % protease solution (Unmasking fluid P, Art.-No. DE110) for 15 min at RT, improvement of staining may be achieved with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000). Incubation Time: 60 min at RT
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human epidermis using pan Cytokeratin antibody