Pan-Cadherin Antibody

• Catalog Number: orb1822675
• Category: Antibodies
• Description: Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as beta-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin 6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Pan-CAD/8020
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant fragment of human C-terminal of Pan-Cadherin was used as the immunogen for the Pan-Cadherin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Pan-Cadherin antibody is available for research use only.
• UniProt ID: P33151, P12830, P22223, P55283, P19022
• Storage: Aliquot the Pan-Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human skin tissue with Pan-Cadherin antibody (clone Pan-CAD/8020). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma tissue with Pan-Cadherin antibody (clone Pan-CAD/8020). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Pan-Cadherin antibody (clone Pan-CAD/8020) as confirmation of integrity and purity.