Pak1 Antibody

• Catalog Number: orb1273558
• Category: Antibodies
• Description: Pak1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of rat p21 activated kinase 2 (PAK-2). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.
• Concentration: batch dependent
• Dilution range: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68/70
• Target: Pak1
• UniProt ID: P35465
• NCBI: P35465
• Storage: PAK1 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
• Note: For research use only
• Application notes: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Expiration Date: 12 months from date of receipt.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1, 2, 3 antibody. The immunolabeling of PAK is completely eliminated by treatment with lamda-Ptase.